Physical Modification of Agar: Formation of Agar-fatty Acid Complexes

Comparison of Paraffin Bait, Humic Acid Vitamin B Agar and Paraffin Agar Methods to Isolate Nocardia from Soil

Abstract Background and Objective: The Isolation of Nocardia species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for Nocardia species. Material and Met...

Eosin methyl-green sulfite agar; a modification of Levine's E.M.B. agar.

The Introduction of Agar-agar into Bacteriology.

Among fundamental developments in bacteriology the elaboration of solid culture substrates will always hold first place. With the introduction of a method for isolating micro6rganisms and growing them in pure culture, bacteriologists possessed the means for studying what Ferdinand Cohn (1872) called "Die kleinsten lebenden Wesen." Credit for the discovery of the solid medium technique is common...

The soft agar colony formation assay.

Anchorage-independent growth is the ability of transformed cells to grow independently of a solid surface, and is a hallmark of carcinogenesis. The soft agar colony formation assay is a well-established method for characterizing this capability in vitro and is considered to be one of the most stringent tests for malignant transformation in cells. This assay also allows for semi-quantitative eva...

Differentiation of Candida dubliniensis from Candida albicans on staib agar and caffeic acid-ferric citrate agar.

The methods currently available for the identification of the pathogenic yeast Candida dubliniensis all have disadvantages in that they are time-consuming, expensive, and/or, in some cases, unreliable. In a recent study (P. Staib and J. Morschhäuser, Mycoses 42:521-524; 1999) of 14 C. dubliniensis and 11 C. albicans isolates, it was suggested that the ability of C. dubliniensis to produce rough...